fetching a lysogen phage
Dominic-Luc Webb molmed
domweb at mbox.ki.se
Tue Aug 7 15:32:52 EST 2001
Has anyone tried to fetch a phage from a
commercial lysogen like Novagen BL21(DE3)
E coli (T7 RNApol)?
For that matter, has anyone tried to fetch
the pLysS (plasmid??) giving the lysozyme
and chloramphenicol resistance from the
I had occasion to do the above recently
and the latter (pLysS), at least, was a
failure, just growing and doing a miniprep.
No plasmid could be found at all.
I could see that maybe the lysogen will not
ever make a complete new phage again, but I
am not sure what happened to the vector for
lysogen and chloramphenicol that it can't
be fetched from a miniprep.
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