A way of measuring bacterial activity? Ideas?

Halogen halo.&anti.spam&.gen at tpg.com.au
Sun Aug 12 00:43:15 EST 2001


Basically I have an undergraduate project to test this new idea for a
bioreactor.

Does anyone know a simple way of quantitatively measuring a product produced
by bacteria without resorting to using equipment like HPLC?

I've tried measuring H2S produced by sulphate reducing bacteria, but this is
difficult to work out since it's only practical to measure it as a gas and a
lot is staying dissolved in the liquid in the bioreactor.
I've tried using pigment produced by psuedomonas fluorescens, but this
didn't work due to this bacteria only producing pigment under certain
conditions.

Any other ideas?  I'm really looking for a bacteria I can easy assay a
metabolic product of using a colour reaction and a spectrophotometer, but I
can't seem to find a way to do this anywhere.

Thanks,








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