..fungal titers?

John Gentile yjgent at home.com
Mon Feb 19 12:32:29 EST 2001


A niger is a bit dangerous to be doing this kind of thing. You don't
quantitate hyphal fungi in the same way you do for yeasts and bacteria. Your
plate or tube will probably have one large colony with billions of spores
just waiting to be released into the air. A. niger has been know to cause
infections. I think you need to re-read your protocol or ask your prof or
teacher about this. I don't like to play with molds any more than I have to.

-- 
John Gentile                                    Rhode Island Apple Group
yjgent at home.com                                      President
 "I never make mistakes, I only have unexpected learning opportunities"

> From: nmaccallister at webtv.net
> Organization: WebTV Subscriber
> Newsgroups: bionet.microbiology
> Date: Mon, 19 Feb 2001 00:17:48 -0800 (PST)
> Subject: ..fungal titers?
> 
> I'll be growing out A. niger and C. albicans next week,  hoping to
> obtain 10^6 titers.  Any firm preferences out there re growing in Sab
> broth as oppossed to a buffer overlay of plate growths and manual
> dislodgement?  
>> Can a 3 day Sab broth culture at 22.5 be               bettered?
>> Would 25* (or 27*) provide better titers?
>> Would that require antibiotic to minimize
> bacteria?  
> Or how about,.. grow them out on plates,.. overlay and dislodge,.. and
> then refrigerate that supernatant,.. using an ml or two when needed.
> Anybody know a good reference in this area? Thanks!
> 






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