Microbiology List: FW: MILK agar?

Robert Friedel Rfriedel at perrittlab.com
Thu Feb 22 12:44:05 EST 2001


1) Skim Milk Agar- pH 7.0 +/- 0.1

Agar 15.0 grams
Pancreatic Digest of Casein 5.0 grams
Yeast Extract 2.5 grams
Glucose 1.0 grams
Add components except Skim Milk Solution. 

2) Skim Milk Solution 100 milliliters
Add skim milk solids to purified water and bring volume to 100 milliliters.
Mix and autoclave for 15 minutes at 121C/15 psi.  Cool to 45-50C.

3) Aseptically add 100 milliliters of cooled Skim Milk Solution to (1).

Atlas, R. M. 
Handbook of Microbiological Media, 1993

Bob Friedel
Quality Assurance Manager
Perritt Laboratories, Inc. 
rfriedel at perrittlab.com

-----Original Message-----
From: Hochberg, Alan M [mailto:Hochbeam at Qualicon.email.dupont.com]
Sent: Thursday, February 22, 2001 7:52 AM
To: micro-list at qualicon.com
Subject: Microbiology List: FW: MILK agar?


From:	J.Rowlands [mailto:oss004 at bangor.ac.uk]
<mailto:[mailto:oss004 at bangor.ac.uk]> 
Sent:	Thursday, February 22, 2001 6:43 AM
To:	microbio at hgmp.mrc.ac.uk <mailto:microbio at hgmp.mrc.ac.uk> 
Subject:	MILK agar?

G'day all,

I've a student trying to get to grips with milk agar for a protease test.
It's a long time since I did this!  References suggest autoclaving
milk-powder solution, but this seems dooomed to failure with heat
denaturation.  
Anyone currently engaged successfully in milk agar that can give advice will
become our lab hero for the week!
				Many thanks,
John Rowlands,
University of Wales, Bangor.
-- 
------------ 
J.Rowlands         oss004 at bangor.ac.uk <mailto:oss004 at bangor.ac.uk> 


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