Methanol Fixation

John Gentile yjgent at home.com
Thu Jul 19 21:07:47 EST 2001


We usually use a warming plate (actually a slide dryer) that is set at about
65 C. However for our positive blood culture smears we use a 1 minute
methanol fix before GS.

A couple of weeks ago we had a case of Haemophilus in a blood culture and it
was almost impossible to find any organisms in the smear - with or without
the methanol fix. 
-- 
John Gentile                                    Rhode Island Apple Group
yjgent at home.com                                      President
 "I never make mistakes, I only have unexpected learning opportunities"

> From: "Joyce & Tim" <glenvale at home.com>
> Organization: Excite at Home - The Leader in Broadband http://home.com/faster
> Newsgroups: bionet.microbiology
> Date: Thu, 19 Jul 2001 05:36:51 GMT
> Subject: Methanol Fixation
> 
> Does anyone have any information or a procedure for methanol fixation in
> bacterial smear preparation. I understand this technique produces less
> damage during prep since heating with a burner or incinerator is not
> required.
> 
> T. Burger
> Dept. of Biological Sciences
> Scott Community College
> 500 Belmont Road
> Bettendorf, IA 52722
> 
> tburger at eiccd.cc.ia.us
> 
> 




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