Pseudomonas and pH
mw132 at mole.bio.cam.ac.uk
Mon Jul 30 08:17:10 EST 2001
my aim is to pull down Pseudomonas aeruginosa using an antibody to
an outer membrane protein and protein A Sepharose beads. Then I want to
disrupt the attachement to the beads and plate the cells on LB plates. I
know that glycine buffer pH3.0 will separate IgG and protein A, BUT will
Pseudomonas aeruginosa survive this?
Is this a good or a dumb idea? Regards, Mike.
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