transformation of Bacillus

Dr. Artem Evdokimov eudokima at mail.ncifcrf.gov
Sun Jun 10 14:35:44 EST 2001


Are you sure that your DNA is compatible ? What I mean is - perhaps it
is so toxic that all the transformed cells croak ?

Have you tried transforming your DNA into other bacteria, just as a
control ?

Just a thought.

A.

"R. Jayakumar" wrote:
> 
> hi..
>    I badly need some help here.  I have been trying my best to transform my
> bacillus isolate with a plasmid construct.  The isolate looks like a new
> strain of Bacillus subtilis.  But I need to put my construct into it.  I
> have tried natural competency method, electroporation etc..and even
> triparental mating.  But to no avail.  The strain stubbornly refuses to
> accept the DNA>  Can any good samaritan give me some ideas on how to go
> about it.  I will be eternally grateful to him.
>    thank you
> sincerley
> jayakumar
> 
> ////////////////////////////////////////////////////\\\\\\\\\\\\\\\\\\\\\\\\
> \\\\\\\\\\\\\\\\\\\\\\\\\\\
> R. Jayakumar
> Dept. of Molecular Microbiology,
> School of Biotechnology,
> Madurai Kamaraj University,
> Madurai - 625021. India.
> tel: +91-452-859141(night hours), +91-452-858471 (ask to connect to 374)
> fax:+91-452-859105
> Efax: (603)-688-4665
> Email: jakkku at mrna.tn.nic.in, (send a copy to jakku at zxmail.com ....just in
> case)
> ////////////////////////////////////////////////////\\\\\\\\\\\\\\\\\\\\\\\\
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> Science is constructed of facts like a house is of bricks, but a mere
> accumulation of facts does not make science nor does a pile of bricks a
> house
> -Henri Poincare
> 
> ---

-- 
|Dr. Artem Evdokimov   Protein Engineering |
| NCI-Frederick        Tel. (301)846-5401  |
|              FAX (301)846-7148           |
|        eudokima at mail.ncifcrf.gov         |
|      http://www.ncifcrf.gov/plague       |




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