Creating recA- mutant of a K-12 Strain (JM83)

Dr. Duncan Clark news at hgmp.mrc.ac.uk
Thu Jun 21 03:17:05 EST 2001


In article <3B30C294.C467F4AF at charite.de>, the eminent Matthias Paschke
at BIOSCI/MRC Human Genome Mapping Project Resource Centre wrote
>I need a protocol of how to make a recA- or recA1 mutant starting with
>JM83.

Look for papers by Messing on construction of JM strains and M13's maybe
10-20 years ago in Gene. Complete protocols there.

Duncan
-- 
It might look like I'm doing nothing, but at the cellular level I'm really
quite busy.

Dr. Duncan Clark
GeneSys Ltd.
Tel. +44 (0) 1252376288
FAX +44 (0) 8701640382




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