Problems isolating plasmids from E.coli JM110

Dr. Duncan Clark d_clark at nospam.demon.co.uk
Mon Mar 5 05:59:31 EST 2001


In article <3AA12B91.A2082E87 at online.no>, the eminent Trond Erik Vee
Aune at Norwegian university of science and technology wrote
>All the plasmids are low-copy, but the ones I have trouble with have a
>copy-up mutation that makes the copy number about 10 times higher than
>the ones I have managed to isolate from.

I've used JM110 over the years without problems. There is a endA mutant
available I think from Stratagene as SCS110 or something similar.

If you are just after a dcm methylation free plasmid then try any E.coli
B strain.

Duncan
-- 
The problem with the gene pool is that there is no lifeguard.

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk






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