Problems isolating plasmids from E.coli JM110

Trond Erik Vee Aune teva at online.no
Mon Mar 5 11:17:20 EST 2001



Sergio wrote:

> Have you checked whether the coli is actually harbouring the plasmid?

We grow it with antibiotic selection. So it shouldn't grow unless it had the
plasmid.

> Try a colony miniprep to verify you have enough plasmid before performing the
> miniprep, although this technique doesn't work very well with low copy plasmids.

What is 'colony miniprep'? Just a direct prep on the colony without inoculation
first to liquid media? In that case I really doubt I would get any plasmid since my
plasmids are very low-copy.

Trond Erik







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