Tim Chance tim.chance at
Fri Mar 16 15:56:05 EST 2001

Both Bacillus (aerobic) and Clostridium (anaerobic) produce endospores and
just to be awkward often appear Gram-negative. They are very easily
decolourised. If you are using acetone to decolourise your Grams be very
careful or try something like methanol instead. I'm not sure you would grow
C.burnetii even if it was given in a practical. Good luck.


paint52 <paint52 at> wrote in article
<ucNk9ikqAHA.277 at cpmsnbbsa07>...
> Coxiella burnetti (Q fever) produces endospore-like structures.
> <Aimee023 at> wrote in message news:da.36fa3ac.27dbe4b4 at
> Hi,
> I'm a Bacteriology student working on an unknown experiment.  We were to
> isolate 2 unknown organisms and determine their identity down to Genus
> Species. I have had no problem getting the 2 organisms isolated and
> successfully.  Here is my problem....I did several Gram Stains on
Organism B
> and it is definitely Gram Negative, the rods are short but there are
> endospores!!  I did 2 endospore tests and my TA also concluded that I had
> endospore formation.  I didn't think Gram Negative bacteria could produce
> endospores. My TA cannot give me any additional information because this
> an independent assignment.  There is a list of possible organisms and my
> unknown B doesn't fit any of these.  Can anyone provide
> suggestions/advice...I would greatly appreciate it =)

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