bacterial spores to vegetative cells

Aldert Zomer zomeral at
Mon Mar 19 11:15:32 EST 2001

Hi all,

We're having some minor problems with the growth of vegetative cells
from spores. We treat the spores with a heat shock, 85 oC for 5 minutes
to induce growth but sometimes the spores just don't grow. (The spores
in question are from clostridium beyerinckii and clostridium
tyrobutyricum, found in cheese)

Are there any other methods to induce the spores?

Aldert Zomer

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