Microbiology List: culture contamination

Rupal Deva rupal at cipmail.ukbf.fu-berlin.de
Sat Mar 31 06:57:11 EST 2001


I aswell agree that it could probabbly be mycoplasma whic is a common lab
contaminent in cell culture.
the only way to get probable rid of is treatment with BM cyclin.
i had similar problem. it can be identified by pcr detection.
best wishes 

On Fri, 30 Mar 2001, Carter Timothy B wrote:

> From: maya [maya-khorchide at bigfoot.de]
> Sent: Sunday, March 25, 2001 4:54 AM
> To: ageing at hgmp.mrc.ac.uk; bio-info at hgmp.mrc.ac.uk;
> bioforum at hgmp.mrc.ac.uk; cellbiol at hgmp.mrc.ac.uk; immuno at hgmp.mrc.ac.uk;
> methods at hgmp.mrc.ac.uk; microbio at hgmp.mrc.ac.uk;
> neur-sci at hgmp.mrc.ac.uk; proteins at hgmp.mrc.ac.uk
> Subject: culture contamination
> I am working with cell cultures and it seems that I have a contamination
> problem.
> Under light microscopy dark dots are visible in the cells and are likely
> to be also moving on their surface. Cell growth, attachment and
> viability are affected.
> These mysterious creatures are only observed in association with cells
> but not in incubated cell free medium.
> Years ago only some cultures were involved but now the infection is
> worsening and spreading about other cultures.
> If you have an idea who these intruders could be or have an advice how
> to recognize them or get rid of them please help me.


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