coliform testing

Dave david.lawton1 at spamfreezonentlworld.com
Thu Nov 15 13:44:45 EST 2001


"Liz Asple" <lasple at bio.ie> wrote in message
news:9t0rk6$e61$1 at kermit.esat.net...
> Hello,
>
> I'm actually looking for an easy and specific procedure to find out any
> coliform contamination in bacterial mixtures containing different kinds of
> microorganisms (total concentration 10*8 bacteria/ml) mixed together.
>
Try a more inhibitory Maconkey formulation or something like Chromocult from
Merck.
If you're using a wide definition of "coliform" i.e. Enterobacteriaecae
then on chromocult E. coli grow a purple/blue colonies while other coliforms
are red.

An alternative could be violet red glucose bile agar, all coliforms are red.

Both agars are pretty specific

Dave





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