denaturating agarose gel electrophoresis

gavin gavin at zonvark.wustl.edu
Sat Nov 17 09:13:24 EST 2001


I would try books on physical biochemistry.  One that I came across recently was:
Physical Biochemistry, Principles and Applications by David Sheehan
(ISBN 0471986631).  It has a chapter devoted to the physical basis of
electrophoresis (various kinds). Yes, it has a good list of references.

Gavin Chan
Earth & Planetary Sciences        Tel: (314) 935-4080
Washington University             Fax: (314) 935-7361
Campus Box 1169                 email: gavin at zonvark.wustl.edu
One Brookings Dr.                 www: http://levee.wustl.edu/~chan
St. Louis, MO 63130-4899


Urs Lewandrowski wrote:

> Hi,
>
> I´m looking for the chemical principle behind denaturating formaldehyde gel
> electrophoresis (agarose gels for RNA identification). Does anyone know, how
> exactly the formaldehyde interacts with RNA or where the denaturating effect
> comes from? A link to tech. lit (primary literature) would be best!
>
> Thanks
>
> Urs Lewandrowski
> urs.lewandrowski at ruhr-uni-bochum.de




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