can RK2 and SC101 replicon type plasmids replicate in same E. coli strain?

Duncan Clark junk at hgmp.mrc.ac.uk
Thu Apr 18 07:14:48 EST 2002


Historians believe that in newspost <3CBE70FC.7711AEFD at utu.fi> on Thu, 
18 Apr 2002, Pekka Virtanen <pelavi at utu.fi> penned the following 
literary masterpiece:
>Hello
>
>I need to consrtuct an E. coli strain which harbours two plasmids: the
>other has SC101 type replicon and the other RK2 type replicon. Can these
>replicate in the same host strain?
>
>Pekka Virtanen
>
>---

As far as I can remember they are different so you should be OK. One way 
to find out ...

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get
sliced ....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382




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