source for E.coli NB42

Steve sstod at family-net.net
Wed Aug 14 19:52:45 EST 2002


Dr. Blaschke,

I'm no expert on E.coli strains, but you raised my curiosity so I did
some searching.  Here's what I found.

NB42 as an indicator strain for water quality monitoring in Sydney:
http://www.sydneywater.com.au/html/AER2000/html/qual_inland/hawkes_berowra_bact.htm  


Found it in the following HTML document.  Perhaps Y.Q.GOSSER etal. would
reveal a source:

HEADER    KINASE                                  03-OCT-97   1AWO
TITLE     THE SOLUTION NMR STRUCTURE OF ABL SH3 AND ITS RELATIONSHIP
TITLE    2 TO SH2 IN THE SH(32) CONSTRUCT, 20 STRUCTURES
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: ABL TYROSINE KINASE;
COMPND   3 CHAIN: NULL;
COMPND   4 FRAGMENT: SRC-HOMOLOGY 3 (SH3) DOMAIN;
COMPND   5 EC: 2.7.1.112;
COMPND   6 ENGINEERED: YES;
COMPND   7 MUTATION: N64S, N120S
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE   3 ORGANISM_COMMON: HUMAN;
SOURCE   4 PLASMID: PGEX;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   6 EXPRESSION_SYSTEM_STRAIN: NB42;
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: PGEX;
SOURCE   8 OTHER_DETAILS: EXPRESSED AS GST FUSIONS AND CLEAVED
KEYWDS    KINASE, SH3 DOMAIN, TRANSFERASE, PHOSPHOTRANSFERASE,
KEYWDS   2 PROTO-ONCOGENE, MULTIPLE DOMAIN, LEUKEMIA
EXPDTA    NMR, 20 STRUCTURES
AUTHOR    D.COWBURN
REVDAT   1   28-JAN-98 1AWO    0
JRNL        AUTH   Y.Q.GOSSER,J.ZHENG,M.OVERDUIN,B.J.MAYER,D.COWBURN
JRNL        TITL   THE SOLUTION STRUCTURE OF ABL SH3, AND ITS
JRNL        TITL 2 RELATIONSHIP TO SH2 IN THE SH(32) CONSTRUCT
JRNL        REF    STRUCTURE (LONDON)            V.   3  1075 1995
JRNL        REFN   ASTM STRUE6  UK ISSN 0969-2126                 2005


Good Luck,
Steve Stoddard


Ulrich Blaschke wrote:
> 
> Does anyone has informations about the E.coli strain NB42.
> A commercial source of the strain would be extremly helpful.
> 
> thanks, Ulrich
> 
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