source for E.coli NB42

Richard Oeffner roeffner at csusm.edu
Thu Aug 15 13:01:10 EST 2002


You might also want to search the ATCC website, i think it is www.atcc.org.
There is also a culture collection in Europe ... I think it is DSMZ, but it is
in Germany I believe. These are places to order bacterial strains, etc. They
might help you find out more about your question.

Richard

Emir Khatipov wrote:

> I was also curious, and did the search myself after I saw the initial post.
> However, no clue where people get this strain from. I would just contact
> authors of original papers who used the strain. Check Pubmed
> (www.ncbi.nlm.nih.gov ), as well as protein structure section at NCBI: some
> proteins were expressed in NB42 for crystallization. My guess is it is
> Neatene's NovaBlue strain, but there is no reference on their website. Maybe
> worth calling Novagen as well. The Australian link seems to be irrelevant:
> it's just a coincidental designation.
> Emir
>
> "Steve" <sstod at family-net.net> wrote in message
> news:3D5AFB5D.85F10C51 at family-net.net...
> > Dr. Blaschke,
> >
> > I'm no expert on E.coli strains, but you raised my curiosity so I did
> > some searching.  Here's what I found.
> >
> > NB42 as an indicator strain for water quality monitoring in Sydney:
> >
> http://www.sydneywater.com.au/html/AER2000/html/qual_inland/hawkes_berowra_b
> act.htm
> >
> >
> > Found it in the following HTML document.  Perhaps Y.Q.GOSSER etal. would
> > reveal a source:
> >
> > HEADER    KINASE                                  03-OCT-97   1AWO
> > TITLE     THE SOLUTION NMR STRUCTURE OF ABL SH3 AND ITS RELATIONSHIP
> > TITLE    2 TO SH2 IN THE SH(32) CONSTRUCT, 20 STRUCTURES
> > COMPND    MOL_ID: 1;
> > COMPND   2 MOLECULE: ABL TYROSINE KINASE;
> > COMPND   3 CHAIN: NULL;
> > COMPND   4 FRAGMENT: SRC-HOMOLOGY 3 (SH3) DOMAIN;
> > COMPND   5 EC: 2.7.1.112;
> > COMPND   6 ENGINEERED: YES;
> > COMPND   7 MUTATION: N64S, N120S
> > SOURCE    MOL_ID: 1;
> > SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
> > SOURCE   3 ORGANISM_COMMON: HUMAN;
> > SOURCE   4 PLASMID: PGEX;
> > SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
> > SOURCE   6 EXPRESSION_SYSTEM_STRAIN: NB42;
> > SOURCE   7 EXPRESSION_SYSTEM_PLASMID: PGEX;
> > SOURCE   8 OTHER_DETAILS: EXPRESSED AS GST FUSIONS AND CLEAVED
> > KEYWDS    KINASE, SH3 DOMAIN, TRANSFERASE, PHOSPHOTRANSFERASE,
> > KEYWDS   2 PROTO-ONCOGENE, MULTIPLE DOMAIN, LEUKEMIA
> > EXPDTA    NMR, 20 STRUCTURES
> > AUTHOR    D.COWBURN
> > REVDAT   1   28-JAN-98 1AWO    0
> > JRNL        AUTH   Y.Q.GOSSER,J.ZHENG,M.OVERDUIN,B.J.MAYER,D.COWBURN
> > JRNL        TITL   THE SOLUTION STRUCTURE OF ABL SH3, AND ITS
> > JRNL        TITL 2 RELATIONSHIP TO SH2 IN THE SH(32) CONSTRUCT
> > JRNL        REF    STRUCTURE (LONDON)            V.   3  1075 1995
> > JRNL        REFN   ASTM STRUE6  UK ISSN 0969-2126                 2005
> >
> >
> > Good Luck,
> > Steve Stoddard
> >
> >
> > Ulrich Blaschke wrote:
> > >
> > > Does anyone has informations about the E.coli strain NB42.
> > > A commercial source of the strain would be extremly helpful.
> > >
> > > thanks, Ulrich
> > >
> > > ------------------------------------------------
> > > Dr. Ulrich K. Blaschke
> > > november AG                               Tel   ++49 9131 75088-423
> > > Ulrich-Schalk-Str. 3                      FAX           ++49 9131
> 75088-439
> > > 91056
> > > Erlangen                            email         blaschke at november.de
> > > Germany                                   web           www.november.de
> > > ------------------------------------------------
> > >
> > > november AG, Gesellschaft für Molekulare Medizin, Ulrich-Schalk-Str. 3,
> > > D-91056 Erlangen/Germany
> > > Tel: +49 (0) 9131 / 750 88 - 0, Fax: +49 (0) 9131 / 750 88 - 99
> > > e-mail: info at november.de,   www.november.de
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> > > Aufsichtsrat: Dr. Gerald Möller (Vorsitzender), Ingolf Knaup, Ulrich
> Kraft,
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> > > ---
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