acetobacter xylinum growth rate?

John Gentile yjgent at cox.net
Wed Aug 28 20:31:26 EST 2002


Just thinking off the top of my head. How about:

1 - set up a simple broth culture with a known amount of organism. Proof can
be made with a simple colony count of a measure amount spread on an agar
plate.

2 - incubate at the optimum temp (or even use different temps). and perform
colony counts at different intervals.

3 - plot the results and calculate the growth rate and the generation time.

The colony counts would have to be done on serial dilutions until you can
get a countable number of colonies.

-- 
John Gentile                            President,  Rhode Island Apple Group
yjgent at cox.net                      RIAG Web page:  www.wbwip.com/riag/
"I never make mistakes, I only have unexpected learning opportunities!"



> From: gerchman at Princeton.EDU (Yoram Gerchman)
> Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
> Newsgroups: bionet.microbiology
> Date: 28 Aug 2002 21:11:05 +0100
> Subject: acetobacter xylinum growth rate?
> 
> Greetings netters
> does anyone know of a simple way (to be used in a high school lab) to
> estimate growth rate of acetobacter xylinum?
> Thanks Yoram
> ---------------------------------------------------------
> "Support bacteria, it's the only culture some people have."
> Anonymous
> 
> ---




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