acetobacter xylinum growth rate?

Lesley Robertson l.a.robertson at tnw.tudelft.nl
Thu Aug 29 03:09:34 EST 2002


"Emir Khatipov" <khatipovNO at NOuchicago.edu> wrote in message
news:A1gb9.25$a5.6616 at news.uchicago.edu...
> Or maybe you could use spectrophotometry at 540-620nm to measure OD of the
> liquid culture. I believe Acetobacter xylinum can grow in clear liquid
> media.
> Emir
>
Yes, that's what I would do. You don't need a big spectrophotometer, any
simple machine capable of measuring optical density of liquids would do....
Now I'm getting nostalgic for the old Klett apparatus. If you wanted
something slightly more complicated, and the bug will grow in a mineral
salts medium (haven't used it), you could get them to take a few samples and
measure the substrate consumption rate and increase in biomass (either by
dry weight or by protein) to confirm that they're not seeing precipitation.
Lesley Robertson
http://www.beijerinck.bt.tudelft.nl






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