Clostridium difficult

Rottjakob, Dave dave.rottjakob at
Mon Jan 28 16:08:40 EST 2002

I am looking for information on the best way to dry clostridium onto a
glass carrier and assure recovery in the neighborhood of 10^4. Any
ideas.  I have started with a 10^8 inoculum and dried @ 36 C for 40
minutes in an aerobic environment and have not recovered anything. I am
afraid that the condensation seen in an anaerobic environment would
prevent inoculum from drying on the carrier.  I would eventually like to
do a germicidal spray test.

Dave Rottjakob
Microbiology Project Manager
AppTec Laboratory Services
2540 Executive Dr.
St. Paul, MN 55120
dave.rottjakob at


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