I would definitely use a buffer. I would first determine pH optimum of
catalase anyway, and then do all the subsequent experiments at that pH.
However, there it is quite conceivable that metal ions can shift pH optimum,
too. So I would not ignore investigating pH dependence in presence of
"vashattack" <vashattack at yahoo.co.uk> wrote in message
news:3c8e4ee5.10723849 at news.freeserve.co.uk...
> I hope this is not off topic and if it is I apoligise.
> I am currently doing a project on the effects of different metal salts
> on the effects of catalase breaking down Hydrogen Peroxide and was
> wondering whether a buffer solution is needed or not as I am not
> investigating effects of pH.
> I would be very greatful for anyones help.
> Thank You