S. aureus integration vector pCL84

Pekka Virtanen pelavi at utu.fi
Wed Mar 27 08:18:33 EST 2002


Hello

I have problems with the integration vector pCL84. Has anyone used this
vector with success? I have tried to get this system to work, but I
haven't got any colonies from the electroporation of RN4220 with
pYL112delta19. Not even with the plain vector. Do you have any helpful
hints for me?

Pekka Virtanen
Ph.D student
University of Turku, Finland
pelavi at utu.fi

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