Aneal two long single strand complementary primers

Isaac Xin Pei xp12 at columbia.edu
Fri Oct 4 10:52:32 EST 2002


Hi, Molecular Biologists,

I have designed a long sequence tag about 70mers; after obtaining the DNA
primers, now I need to anneal them, and ligate them to my vector. Does
anyone has a protocol for annealing the long single-complentary strand DNA
for these works? Thanks a lot!

Best, Isaac




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