l.a.robertson at tnw.tudelft.nl
Mon Oct 14 03:08:09 EST 2002
"Ted Jones" <sirbooli at comcast.net> wrote in message
news:-A6dne1wQKJrlzqgXTWc2Q at News.GigaNews.Com...
> i was wondering if there was a standardized chart for bacteria/DH5a OD600
> with corresponding cell concentration (x cells/ml). can i use the one for
> haploid yeast. yes i know there is a hard way by serial dilutions and ect
> but i just wanted to know if there was anything published and readily
Apart from anything else, if you think about it, yeast are much bigger and
you get less of them to the OD unit than bacteria. I wouldn't even trust one
made for a different bacterium - long thing bacteria will give different
result than short fat one. Moreover, the growth conditions will affect the
results - if the cells are full of PHB for example, you'll get a higher OD
per cell than you should.
By the time you finish mucking around, it would be quicker to do it
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