DNA-fingerprint

John M Neary neary at acsu.buffalo.edu
Sat Sep 28 17:05:56 EST 2002


Rudy,

We currently use pulsed-field gel electrophoresis (PFGE)to resolve bands of
genomic DNA cut with a rare-cutting restriction enzyme (usually SmaI).  You
may also want to look into multi-locus sequence typing (MLST), which is a
little more involved, but less ambiguous and easy to reproduce.  A PubMed
search should provide plenty of info on these techniques.

John

"badman" <badman at sky.be> wrote in message
news:ok3cpu03eikppsaqaj8e3atenfqrb28lqo at 4ax.com...
> Can someone help me with a good script for DNA-fingerprinting? I am
> looking to implemnet the technique in the lab and I am looking for a
> good procedure. I want to do DNA-fingerprinting for Legionella
> pneumophila; Can someone help me to find the just inforamtion?
>
> thnak you
>
> rudy





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