DNA-fingerprint

Duncan Clark junk at hgmp.mrc.ac.uk
Sun Sep 29 06:05:24 EST 2002


Historians believe that in newspost 
<an595j$c3b$1 at prometheus.acsu.buffalo.edu> on Sat, 28 Sep 2002, John M 
Neary <neary at acsu.buffalo.edu> penned the following literary 
masterpiece:
>Rudy,
>
>We currently use pulsed-field gel electrophoresis (PFGE)to resolve bands of
>genomic DNA cut with a rare-cutting restriction enzyme (usually SmaI).  You
>may also want to look into multi-locus sequence typing (MLST), which is a
>little more involved, but less ambiguous and easy to reproduce.  A PubMed
>search should provide plenty of info on these techniques.
>
>John
>
>"badman" <badman at sky.be> wrote in message
>news:ok3cpu03eikppsaqaj8e3atenfqrb28lqo at 4ax.com...
>> Can someone help me with a good script for DNA-fingerprinting? I am
>> looking to implemnet the technique in the lab and I am looking for a
>> good procedure. I want to do DNA-fingerprinting for Legionella
>> pneumophila; Can someone help me to find the just inforamtion?
>>
>> thnak you
>>
>> rudy
>
>

RAPD or 16s/23s intergenic region PCR plus RE digestion etc. ?

Must be easier than PFGE :-))

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.



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