rmaldonado at REMOVEua.es
Mon Feb 17 14:47:32 EST 2003
Philipp Wechner wrote:
> why do i only get these big colonies with BL21?
It is a fairly common thing, in many different bacterial strains, and
depending of the antibiotic used in the selection. I also have observed
it in DH5alpha and HB101. Tetracycline is specially active in making
different size colonies. My guess is that copy number plasmid is
different, for some reason, in those plates. I would not bother about this.
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