weird bacteria - Update
Trond Erik Vee Aune
trondaun at biotech.NOSPAM.ntnu.no
Tue Mar 11 01:57:47 EST 2003
Of course I know all this. Why do you think otherwise?
What I don't understand is:
1) Why do you think I have two strains when it's easier to explain what
I see with one strain than with two?
2) According to your theory the swarmer is not forming colonies under
the current condition, but would, maybe, do this if it is not allowed to
spread out. Why is this?
> It sounds like you're doing micro without a loop or wire to pick
> colonies or something to flame your loop in between touching colonies.
> After you make your 6% agar (or use some differential media as I
> described in another posting) you need to streak for isolation. If you
> obtain two colony morphologies, each colony is a pure culture. You need
> to flame your loop, pick one colony and streak it to another plate. You
> then need to flame your loop again, pick the second colony type, plate
> to a separate plate and streak for isolation. Then flame again.
> This is basic microbiology. If you're using bacteria, even in a genetics
> laboratory, I don't understand how you can do it without all of the
> basic equipment that you need. The ONLY way to separate bacteria is
> streak for isolation, using solid media. You put your inoculum down on
> about 1/3 of the plate. Then you turn the plate a little and streak out
> from this area. You then flame, and then streak another quadrant, then
> turn and streak the last quadrant. You then incubate the plate upside
> down, at least overnight, and look for different colony morphology.
> A visit to your local clinical micro lab will help you immensely! We do
> this EVERY DAY. Gram negative rods don't look THAT different from each
> other upon staining, at least with Gram's stain. Some are smaller and
> thinner or thicker, but that doesn't help you separate them out.
> Judy Dilworth, M.T. (ASCP)
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