SYBR green/propidium iodide staining

emma.carr at bendigo.latrobe.edu.au emma.carr at bendigo.latrobe.edu.au
Thu May 1 20:53:46 EST 2003


I was wondering if anyone had used SYBR green/PI staining to detect dead/live cells in activated sludge samples. Where other people have stained the cells resuspended in some kind of buffer, I would like to air-dry my sludge samples straight onto  gelatin-coated slides and add the stains directly to this. I think that the concentrations of each stain is going to be important, so if anyone has done this and has any advice, I would greatly appreciate it.Thanks

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