fermentation of coli and plasmid stability

EK REMOVEem at NOSPAMunforgettable.com
Thu May 8 13:19:02 EST 2003


You could determine how retention of the plasmid is affected by dilution
rate, substrate concentration, pH, etc., and determine run fermentation at
the optimum rate. Also, you might consider running pH-stat rather than
chemostat, or turbidostat. Sometimes it helps.
-EK

"Ede" <bggfg at gmx.de> wrote in message
news:uf4lbv43h9o8vd7cgnvh2mq5inubsqpvs1 at 4ax.com...
> Hi there,
> is anybody there who can give me an idea, how i can solve the problem
> of low plasmid stability during a fermentation in coli. The problem is
> i cannot select with antibiotics. If somebody knows how i can increase
> the stability of my plasmid it would be great. also if somebody have a
> good review or so about this problem
> Thanks
> Ed





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