Identification og unknown bacterium from Ganges ("wierd bacterium")

Scott Coutts scott.coutts at med.monash.edu.au
Fri May 9 00:46:59 EST 2003


Well, you dont even have to look in places others havent looked... I 
think I mentioned this before, but anyhow - we found one growing as  a 
contaminant on a new agar plate in our cold room!



Trond Erik Vee Aune wrote:
> 
> 
> JEDilworth wrote:
> 
>> Hate to say I told you so........but it just didn't sound like a gram 
>> negative to
>> me.
> 
> 
> Nah, don't feel bad about it. I'm just glad I learnt how to do 16s 
> sequencing. Now I'm going to look for new bacteria, I just need to find 
> some place where other people haven't been looking before.
> 
> Btw, I'm still working with the Ganges bacteria (Paenibacillus alvei). 
> It seem to have a plasmid of less than 30kb, so I'm going to see if I 
> can establish it in some other bacteria and see if some of the phenotype 
> from the Paenibacillus is transferred to the new one, for instance 
> antibiotics production. I need some practical work to do inbetween 
> reading to exam :)
> 
> Trond Erik
> 
> 
>>
>> Bacillus will only sporulate under adverse conditions. If you're 
>> providing it with
>> breakfast, lunch, and dinner on a great plate, it has no need to form 
>> spores.
>>
>> Judy Dilworth, M.T. (ASCP)
>> Microbiology (Clinical) 28 years
>>
>> Trond Erik Vee Aune wrote:
>>
>>
>>> Yes, it was highly motile and swarmed in a very fascinating way. That's
>>> why I decided to identify it. I looked for spores in the microscope, but
>>> couldn't see any, so maybe the cells were in the wrong growth phase.
>>>
>>> Trond Erik
>>>
>>>
>>>>>> Anyway, I got very good match against Paenibacillus alvei. 
>>>>>> According to
>>>>>
>>>>>
>>>>> web-resources this genus do produce different types of antibiotics as
>>>>> well as being able to swarm in different ways
>>>>> (http://ijs.sgmjournals.org/cgi/reprint/49/1/239.pdf). The strange 
>>>>> thing
>>>>> is that it is not a gram negative bug as I thought after staining, but
>>>>> one of you microbiologists suggested that I was a Bacillus strain, 
>>>>> so I
>>>>> guess we geneticists are much better at sequencing than staining ;)
>>>>> Anyway, I'm pretty pleased that I finally got an identification, 
>>>>> but of
>>>>> course a little disappointed that it wasn't a new bacterium
>>>>
>>>>
>>
> 




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