fermentation of coli and plasmid stability

Trond Erik Vee Aune trondaun at biotech.REMOVETHISBEFOREREPLYING.ntnu.no
Fri May 9 01:28:58 EST 2003


You can check out del Solar et al., 1998: "Replication and control of 
circular bacterial plasmids", Bingle and Thomas (2001): "Regulatory 
circuits for plasmid survival". They may be a bit too theoretical but 
they may contain valuable references.

Trond Erik

Trond Erik Vee Aune wrote:
> 
> 
> Ede wrote:
> 
>> Hi there,
>> is anybody there who can give me an idea, how i can solve the problem
>> of low plasmid stability during a fermentation in coli. The problem is
>> i cannot select with antibiotics. If somebody knows how i can increase
>> the stability of my plasmid it would be great. also if somebody have a
>> good review or so about this problem
> 
> 
> You could try using some kind of partition (par) or killer system (i.e. 
> hok/sok). Partition systems (mostly found on low-copy plasmids, I think) 
> make sure that at least a copy of each plasmid is partitioned to each 
> cell at division. Killer systems kill off cells without plasmid. In this 
> way it's better to use a partitioning system than a killer system, since 
> the effect of the last one reduces growth rate.
> 
> I'll look for references when I get to school.
> 
> Trond Erik
> 


-- 
Trond Erik Vee Aune
Department of Biotechnology
Norwegian University of Science and Technology
Email: trondaun at biotech.ntnu.no
http://www.REMOVETHISBEFOREREPLYING!!biotech.ntnu.no/molgen




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