fermentation of coli and plasmid stability

pilly pillarpost at hotmail.com
Fri May 9 14:10:16 EST 2003


Ede <bggfg at gmx.de> wrote in message news:<uf4lbv43h9o8vd7cgnvh2mq5inubsqpvs1 at 4ax.com>...
> Hi there,
> is anybody there who can give me an idea, how i can solve the problem
> of low plasmid stability during a fermentation in coli. The problem is
> i cannot select with antibiotics. If somebody knows how i can increase
> the stability of my plasmid it would be great. also if somebody have a
> good review or so about this problem
> Thanks
> Ed

What plasmid is it? Hi/Lo copy number? And whats on it?
pilly



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