fermentation of coli and plasmid stability

Duncan Clark junk at hgmp.mrc.ac.uk
Mon May 12 04:10:08 EST 2003


Historians believe that in newspost 
<3EBB385C.6080700 at biotech.NOSPAM.ntnu.no> on Fri, 9 May 2003, Trond Erik 
Vee Aune <trondaun at biotech.NOSPAM.ntnu.no> penned the following literary 
masterpiece:
>You could try using some kind of partition (par) or killer system (i.e. 
>hok/sok)

I agree fully. Look for a paper in Biotechnology Vol 6 December 1988 p 
1402-1405 by Kenn Gerdes with hok/sok on a small 580bp cassette. 
Stabilisation factor of 1000 for pBR322 and 10000 for pACYC, p15 ori, 
derivatives

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.



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