Maintaining microaerobic conditions

C Coward cc122 at mole.bio.cam.ac.uk
Wed Nov 5 10:35:34 EST 2003


Greetings microbiologists...We're looking into the possibility of
extending our capacity for growing Campylobacter. Currently we use a
microaerobic workstation to maintain 85% N2/5% O2/5% H2/5% CO2 at 42oC.
We're consdidering getting an air incubator and growing the bugs in
containers with CampyGen gas packs. We've got a couple of microbiology gas
jars but they're an awkward shape so was wondering whether there'd be any
problems with getting appropriate size boxes e.g. Tupperware, taping the
edges to attempt an air-tight seal, and using gas packs inside.

Also, are there any ways (indicator strips for example) to determine if
you've successfully generated a microaerobic environment?

TIA

Chris



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