nobody at elnino.com
Wed Oct 15 15:49:26 EST 2003
"DKafkewitz" <dkafkewitz at aol.com> wrote in message
news:20031015145344.09624.00000978 at mb-m25.aol.com...
> Light scattering varies inversely with wavelength. The shorter the wave
> more the scatter. Hence 440 is more sensitive than 660. But absorbance of
> light by the medium is also important. Colorless media abosrb no visible
> light: that is why they are colorless. Yellow media absorb in the shorter
> range. Therefore you use a longer wave to minimize the false reading of
> Absorbance rather than scattering.
BTW, I always wondered would then it be necessary to use shorter wavelengths
for smaller bacteria? I guess a 300nm monococci would not scatter light at
600nm, whereas 300nm diplococci would. Same would the rod e.g. 200 x 1000
nm. Is my understanding correct?
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