LB media chemical composition

Scott Coutts scott.coutts at med.monash.edu.au
Thu Feb 12 21:03:00 EST 2004


Marcin Imielinski wrote:

> 
> Hello,
> 
> I have a pretty basic question for which I have had difficulty finding
> the answer to ...
> 
> What is the chemical composition of LB media?  The product sheets
> generally list the "ingredients" as tryptone and yeast extract, and some
> list the composition in terms of amino acids and electrolytes.  But it
> appears that given its messy origins this LB should be quite a bit more
> rich with metabolites.  For example does LB contain nitrogenous bases or
> nucleotides?  What carbohydrates are known to be present?
> 

Well, you'd have to look for the chemical analysis of the individual 
'ingredients'. As you say, in the lab, it's made up from trypton, yest 
extract, salt and water. I assume you know what yeast extract and 
trypton actually are? You're not going to get a complete and 
comprehensive chemical analysis because these ingredients are too 
complex. It's not a chemically defined medium!

Scott.




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