Macrophage lysis

Scott Coutts scott.coutts at med.monash.edu.au
Fri Jul 23 06:13:28 EST 2004


Md.Tanvir Rahman wrote:

> Hello,
> 
> What could be the best way to cause lysis of macrophage to release 
> intracellular bacteria [phagocitozed bacteria]. My objective is to extract RNA 
> from phagocitozed bacteria.
> 
> Thanks in advance.
> 
> Rahman
> 
> ---
> 

You could try mild detergent, depending on what type of bacteria you're 
trying to collect. What RNA are you looking for? Does it matter if 
there's RNA from the host cells in there?

Scott.





More information about the Microbio mailing list