Recombinant work with in AT rich bacillus DNA in E. coli

Trond Erik Vee Aune trondaun at biotech.REMOVETHISBEFOREREPLYING.ntnu.no
Thu May 6 01:44:21 EST 2004


We have general problems with cloning and manipulations of Bacillus DNA 
(AT-rich!) in E. coli (DH5 alpha), related to DNA instability, difficult 
to clone, etc. We would be happy to know if anyone knows certain general 
tricks (appropriate E. coli host, growth temperature, DNA preparation, 
etc) that is useful when working with such DNA??

trygve
-- 
Trond Erik Vee Aune
Department of Biotechnology
Norwegian University of Science and Technology
Email: trondaun at REMOVETHISBEFOREREPLYING!!biotech.ntnu.no
http://www.biotech.ntnu.no/molgen

- Must be sad being a dyslectic, agnostic insomniac, lying
   awake during the night, wondering if there really is a dog




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