Recombinant work with in AT rich bacillus DNA in E. coli

Trond Erik Vee Aune trondaun at
Thu May 6 01:44:21 EST 2004

We have general problems with cloning and manipulations of Bacillus DNA 
(AT-rich!) in E. coli (DH5 alpha), related to DNA instability, difficult 
to clone, etc. We would be happy to know if anyone knows certain general 
tricks (appropriate E. coli host, growth temperature, DNA preparation, 
etc) that is useful when working with such DNA??

Trond Erik Vee Aune
Department of Biotechnology
Norwegian University of Science and Technology

- Must be sad being a dyslectic, agnostic insomniac, lying
   awake during the night, wondering if there really is a dog

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