Amplification product size

nmesbah at uga.edu nmesbah at uga.edu
Thu May 27 14:17:55 EST 2004


I am trying to amplify total genomic DNA extracted from soil using archeal 21f and 1392r primers. I expect to get a product ~1400 bp long but I keep on getting small fragments 200-500bp long. Should I extend the extension time?

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