metabolic activity determination
l.a.robertson at tnw.tudelft.nl
Thu Oct 7 09:47:56 EST 2004
"hiren joshi" <DNA_80 at hotmail.com> wrote in message
news:d8ad717.0410070144.3d50e7f9 at posting.google.com...
> hii all i resently join this group and hope will have very good
> intrection oon future.
> i m working on biofilm. i want to know how i can determine metabolic
> state of organism after exposerof it to verious enviromental
> condition. we have confocal microscope so one can sujest me some
> fluresence mediated detection also.
What sort of biofilm? Photosynthetic? Nitrifying? How thick is it? Is it
flat or on sand? The type of organisms will make a big difference in the
type of measurements you make to monitor metabolic activity.
We used microelectrodes (usually pH and oxygen) to monitor activity at
different levels within biofilms, but without a bit more detail, it's hard
to know what to suggest.
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