Colony blot & lysis

H. Boersma f.g.h.boersma at biol.rug.nl
Tue Oct 19 08:05:06 EST 2004


Hello,

i'm planning to do a colony blot on bacteria from soil. Amongst these
bacteria there are of course gram-positive bacteria. I want to grow
these on the nylon filter used for hybridization. the question
involves the lysis of these bacteria. normally NaOH is used. this
won't lysate the gram-positive bacteria. any idea which solution i
should use as lysis buffer?

thanks in advance  
Hidde Boersma PhD student
Groningen
The Netherlands
f.g.h.boersma at biol.rug.nl



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