OD of saturated bacterial culture?
paul_wary at yahoo.com
Wed Sep 8 16:54:49 EST 2004
I am going to express a protein in E. coli host cells (to be precise
BL21 (DE3) cells) and the instructions say that the induction of
protein synthesis through IPTG is to be started at an OD of 0.6, which
is easy enough.
The cells are then grown to saturation before harvesting (no OD given
Can anyone tell me what the approximate OD value of a saturated E.
coli culture is?
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