OD of saturated bacterial culture?

Paul Wary paul_wary at yahoo.com
Wed Sep 8 16:54:49 EST 2004


Hello,

I am going to express a protein in E. coli host cells (to be precise
BL21 (DE3) cells) and the instructions say that the induction of
protein synthesis through IPTG is to be started at an OD of 0.6, which
is easy enough.
The cells are then grown to saturation before harvesting (no OD given
for saturation).
Can anyone tell me what the approximate OD value of a saturated E.
coli culture is?

Paul



More information about the Microbio mailing list