cutting large circular DNA molecules

The Duck theduck at earth.com
Tue Apr 19 12:47:19 EST 2005


Dear All,

I was hoping that you could give me some advice to the following problem:

I would need to cut large circular DNA (several hundreds of kilobases in
size) exactly once (or at most only a few times). I do not know the
nucleotide sequence of the molecules and additionally they will not be the
same. Currently I am trying a number of restriction enzymes, however, this
is presently a workaround. What I would really need is a method that is
cutting these large molecules only once.
I was already thinking of using radioactivity, however, we cannot do this
were I am working due to security constraints.
I would very much appreciate any ideas you could give me.

The Duck.



More information about the Microbio mailing list