[Microbiology] Re: How to calculate the number of transposon mutants for a genomic library?

Bob bbx107 at excite.com.XXXX
Sun Aug 14 11:25:06 EST 2005


On Sun, 14 Aug 2005 00:33:49 -0500, "JXB" <jxb at bellsouth.net> wrote:

>Hi All,
>
>I want to make a transposon library in a gram negative bacteria. The genome
>size in ~1.9 Mb and average gene size is 1 kb. Does anyone know how I
>calculate the theoritical number of transposon mutants I need to knockout
>every gene in this bacteria?
>

Simple approach to start... assume that transposons insert at random
and that genes are all of same size (and that all insertions are
"knockouts"). Then use Poisson distribution to calculate probability
of a gene remaining unhit as a function of average number of
transposons per gene. The real world will undoubtedly make it more
difficult than the calculation suggests, but it gets you started. 

bob


>Thanks,
>
>Jeff Welbank
>jxb at removethis.bellsouth.net
>



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