[Microbiology] Re: Spectro for growth curves

Lesley Robertson l.a.robertson at tnw.tudelft.nl
Mon Jul 4 02:56:22 EST 2005


"Scott Coutts" <scott.coutts at med.monash.edu.au> wrote in message 
news:V4Mxe.12898$oJ.2999 at news-server.bigpond.net.au...
>
> Hi Larry,
>
> Are they the flasks with the sealed tube projecting from the neck? If so, 
> yeah, I'd considered them, but it would be more convenient to have one 
> that allows us to automate the procedure. Since we're buying new specs 
> anyway, I thought it would be best to see if we can get a model that will 
> do the widest range of things we need (: The thing is, I'm pretty sure 
> they exist, I'm just not sure who makes them.
>
There's a few things it would be useful to know - expected volumes, for a 
start. How critical will dissolved oxygen be? Most of the automative reading 
apparatus we had in the past  could only manage a very sedate shaking rate, 
which meant that oxygen was frequently limiting as the cultures matured...
Some years back, I was fortunate to use a spectrophotometer at the Free 
University of Amsterdam which had been adapted to take a full-sized 
continuous culture system (it was used for measuring cytochromes oxidation 
levels at steady state) - 1 litre pot and all. I have an idea that they did 
the adaptions themselves, but I'll try and check - that particular Prof has 
now retired.
Lesley Robertson




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