[Microbiology] Re: Is water considered a decolorizer in Endospore staining?

Jeroen van der Rijst jeroen.van.der.rijst at gmail.com
Tue Jul 26 14:00:10 EST 2005


On Fri, 15 Jul 2005 22:06:43 -0700, bonaserat wrote:

> I have a question regarding the Endospore staining procedure. We had an
> exam in our Microbiology class, and we had to list all functions of
> each of the steps in endospore staining. I did not put water as a
> decolorizer, because it was never listed as such as all the other
> decolorizers for staining as acetone alcohol is for grams stain, and
> acid alcohol for acid-fast stains. I listed malachite green for
> primary, heat as mordant, safranin as the countere stain. Any help or
> links if I am indeed right water is not actually a decolorizer would be
> appreciated. 
> 
> Thanks in Advance.

Hi,

As far as I know is water used to decolorize cells in the the
Schaeffer-Fulton method. The link I added to this message is a Microsoft
Word document I found with Google about the matter. I hope this will clear
things out.

..., 

Jeroen van der Rijst.

http://www2.muw.edu/~lbrandon/Micro/endospore.doc


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