Help an undergraduate student
almahdi at magma.ca
Thu Mar 3 19:00:52 EST 2005
Hello everyone, I truelly and honestly respect and honor your help, and you
have provided me with very (I mean very) interesting ideas. I will discuss
them with my instructor, and if he said "it is not challenging at all"
again, I'll beat him up !! haha.
Again, every one thank you soo much. I will tell you on which topic we will
Have a nice time and good luck with your studies.
"Larry Farrell" <farrlarr at isu.edu> wrote in message
news:1109891845.bb34ea1f4a739d20fa8b565b01e5116a at teranews...
> The_Warrior wrote:
> > Hello every one, I'm a biology student in a University, and I truelly
> > your assistance in choosing a good topic for an undergraduate
> > project.
> Try isolating a bacterial virus (bacteriophage = phage) for your
> favorite bacterium. Simply identify the bacterium to use and identify
> where it can be found in nature (E. coli and sewage samples work very
> well, if you make sure you get settled sewage that has not been
> chlorinated). Collect a sample of that environmental material, suspend
> it is sterile water or use directly if it is an aqueous sample, mix with
> an appropriate volume of 10X broth (of a medium that supports good
> growth of the bacterium to be used)and add a sample of a culture of your
> bacterium. Incubate overnight, centrifuge to remove bacteria, filter
> through a 0.45 micron filter and use the supernate as your phage stock.
> Plate serial dilutions of the stock on your bacterium by the soft agar
> overlay method. Pick isolated plaques and make new stocks of each and
> then compare plaque morphology, replication time for the phage, etc.
> If interested, I can supply additional specifics of the techniques, as
> can many other folks who lurk/post here.
> Larry D. Farrell, Ph.D.
> Professor of Microbiology
> Idaho State University
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