poor polymerization in DGGE

WU Yucheng yucheng_wu at hotmail.com
Sun May 8 02:42:46 EST 2005


Hi everyone,

I am investigating the bacteria diversity in soil by DGGE method.But a
poor polymerization always happened during casting. In fact,the
acrylamide solution doesn't polymerize from below the comb about 1cm to
the top even overnight at temperature(about 20¡æ). The gel oncentration
is 8% and 10ul TEMED and 150ul 10%APS are added to 15ml high or low gel
solution.

Then,who can tell me what is the reason and how I can optimize the
polymerization.

Thank you in advance for your help!!

yucheng wu

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